The Labyrinthine Orb Weaver Spider will conceal itself in the retreat web using dried leaves or other debris to help camouflage it. The spider will sit, legs tucked under and around itself in a tight spot, like a curled leaf, while waiting for insects to become ensnared in the larger web.
Once a prey item is snagged in the web, the Labyrinthine Orb Weaver Spider will go to it, bite it to immobilize it with venom and then proceed to wrap the prey in a cocoon of spider silk where it will remain until the spider wants to consume it.
Adults are active from early spring to late fall. Males stop building and maintaining webs once they mature. Reproduction occurs in rainy spring. Females wrap multiple egg sacs in silk, stranding them together like a string of pearls. This strand of egg sacs is then attached to her web and disguised at debris by putting twigs and leaves around it. She will guard her egg sacs until the spiderlings hatch and jump off her web to start lives of their own.